DNA

Part:BBa_K4115010:Design

Designed by: Renjie Yu   Group: iGEM22_ShanghaiTech_China   (2022-09-21)
Revision as of 02:26, 4 October 2022 by Yurj (Talk | contribs) (→‎Design Notes)

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NSI-US


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 333
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 520
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 333
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 333
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NSI-US and NSI-DS are a pair of commonly used homology arms in S.elongatus.

Source

The sequence is gained from the genome of Synechococcus 2973 [1] and the fragment we use is synthesized by the company.

References

[1] Li, S., Sun, T., Chen, L., Zhang, W. (2021). Designing and Constructing Artificial Small RNAs for Gene Regulation and Carbon Flux Redirection in Photosynthetic Cyanobacteria. In: Basu, C. (eds) Biofuels and Biodiesel. Methods in Molecular Biology, vol 2290. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1323-8_16