DNA

Part:BBa_K4115010:Design

Designed by: Renjie Yu   Group: iGEM22_ShanghaiTech_China   (2022-09-21)
Revision as of 02:18, 4 October 2022 by Yurj (Talk | contribs)


NSI-US


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 333
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 520
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 333
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 333
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NSI-US and NSI-DS is a pair of commonly used homology arms in S.elongatus.


Source

The sequence is gained from the genome of Synechococcus 2973 and the fragment we use is synthesized by the company.

References