Coding
Part:BBa_K4345012:Design
Designed by: Michelle Patricia Group: iGEM22_KU_Leuven (2022-09-30)
FdeR
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 466
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 466
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 466
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 466
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 466
Illegal NgoMIV site found at 653
Illegal NgoMIV site found at 665
Illegal NgoMIV site found at 756 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Source
Engineered LysR-type PfdeAR-FdeR from Herbaspirillum seropedicae
References
De Paepe, B., Maertens, J., Vanholme, B., & De Mey, M. (2018, April 24). Modularization and Response Curve Engineering of a Naringenin-Responsive Transcriptional Biosensor. ACS Synthetic Biology, 7(5), 1303–1314. https://doi.org/10.1021/acssynbio.7b00419