Composite

Part:BBa_K4387003

Designed by: Jana Mehdy, Lea Bruellmann, Marine Mausy   Group: iGEM22_UZurich   (2022-09-30)
Revision as of 22:46, 3 October 2022 by Jmehdy (Talk | contribs)


Negative Control of Nitric Oxide Sensing Genetic Circuit

Usage and Biology

This composite part consists of a superfolder GFP (BBa_K2553008), the NorR regulator (BBa_K4387001), and a double forward terminator (BBa_B0015). To assess the proper induction of our construct by nitric oxide, we removed the pNorVβ promoter to create a negative control. With the removal of the promoter, the presence of nitric oxide should not affect the expression of GFP. Therefore this construct accounts for the leakiness of the constructs BBa_K4387004, BBa_K4387005, BBa_K4387006, and BBa_K4387007.


This construct was tested in the bacterial strain E.coli Nissle 1917.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 439
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None