Regulatory

Part:BBa_K4387000:Design

Designed by: Jana Mehdy, Lea Bruellmann, Marine Mausy   Group: iGEM22_UZurich   (2022-09-28)
Revision as of 21:49, 3 October 2022 by Jmehdy (Talk | contribs) (→‎Source)


Nitric Oxide Sensing Promoter pNorVβ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No codon optimization was necessary. The sequence was taken directly from the reference paper.


Source

The sequence of the pNorVβ was obtained from the paper of Xiaoyu J. Chen et al. [1]

References

[1]Xiaoyu J. Chen, Baojun Wang, Ian P. Thompson, and Wei E. Huang et al. Rational Design and Characterization of Nitric Oxide Biosensors in E. coli Nissle 1917 and Mini SimCells ACS Synthetic Biology 2021 10 (10), 2566-2578 DOI: 10.1021/acssynbio.1c00223