Composite
cex
Part:BBa_K4368001
Designed by: Carmen Jimenez Amores, Alonso Molina Calvo, Paola Morales Gonzalez Group: iGEM22_UMA_MALAGA (2022-09-26)
Revision as of 19:17, 3 October 2022 by Carmeenjmnezz (Talk | contribs)
pcstA + rbs + yebF + cex + terminator
Cex is the gene coding for the exoglucanase enzyme of Celullomonas fimi. In our case, we have incorporated this enzyme into a regulated gene construct under the control of the pcstA promoter. We have also included an export site, yebF, so that this enzyme can be expressed in the cell membrane and thus function correctly together with the enzymes of the CAZymes complex. In addition, the sequence has been optimised in order to incorporate it into the Escherichia coli organism. Finally, a double terminator has been used.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1044
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 268
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 677
Illegal NgoMIV site found at 1050
Illegal NgoMIV site found at 1552 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1097
Illegal SapI.rc site found at 1180
[edit]
Categories
Parameters
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