Composite
cex

Part:BBa_K4368001

Designed by: Carmen Jimenez Amores, Alonso Molina Calvo, Paola Morales Gonzalez   Group: iGEM22_UMA_MALAGA   (2022-09-26)
Revision as of 19:17, 3 October 2022 by Carmeenjmnezz (Talk | contribs)


pcstA + rbs + yebF + cex + terminator

Cex is the gene coding for the exoglucanase enzyme of Celullomonas fimi. In our case, we have incorporated this enzyme into a regulated gene construct under the control of the pcstA promoter. We have also included an export site, yebF, so that this enzyme can be expressed in the cell membrane and thus function correctly together with the enzymes of the CAZymes complex. In addition, the sequence has been optimised in order to incorporate it into the Escherichia coli organism. Finally, a double terminator has been used.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1044
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 268
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 677
    Illegal NgoMIV site found at 1050
    Illegal NgoMIV site found at 1552
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1097
    Illegal SapI.rc site found at 1180


[edit]
Categories
Parameters
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