Composite

Part:BBa_K4115023:Design

Designed by: Kaijun Wang   Group: iGEM22_ShanghaiTech_China   (2022-09-30)
Revision as of 13:50, 3 October 2022 by ParadoxDecade (Talk | contribs) (→‎Design Notes)


J23101-B0034-sfGFP(with LVA)-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed for constitutively expression of sf-GFP protein with degradation tag in E. coli.

Source

Obtained by assembling the corresponding parts in sequence by Gibson Assembly.

References