Composite

Part:BBa_K4115037:Design

Designed by: Kaijun Wang   Group: iGEM22_ShanghaiTech_China   (2022-09-30)
Revision as of 13:30, 3 October 2022 by ParadoxDecade (Talk | contribs) (→‎Source)

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J23107-B0034-SacC-B0015


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 498
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1370
    Illegal PstI site found at 498
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1413
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 498
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 498
    Illegal NgoMIV site found at 757
    Illegal AgeI site found at 479
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed for constitutively expression of SacC protein in E. coli.

Source

Obtained by assembling the corresponding parts in sequence by Gibson Assembly.

References