DNA

Part:BBa_K4342000

Designed by: Jeffrey Chuong and Samer Salman   Group: iGEM22_Austin_UTexas   (2022-10-02)
Revision as of 16:20, 2 October 2022 by Registry (Talk | contribs)

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tdk/kan Cassette

Confers kanamycin resistance and AZT susceptibility. Designed to ligate to Gene Upstream Homology (Type 1) and Gene Downstream Homology (Type 5) via BsaI digestion for ADP1 Genetic Engineering. Used to ligate to P. destructans Target Sequence Upstream Homology (Type 1) and CymR Repressor (Type 56) via BsaI digestion in P. destructans Detector.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 719
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 713
    Illegal XhoI site found at 875
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 719
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 719
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None