Composite
XK
Part:BBa_K4324002
Designed by: Chris Yoo Group: iGEM22_TheKingsSchool_AU_HS (2022-10-02)
lacPO-RBS-XK-T1
This part allows E. coli to express xylulose kinase (XK).
XK is an enzyme that allows the conversion of xylulose to xylulose-5-phosphate (X5P), and vice versa, according to the following chemical equation: D-xylulose + ATP ⇌ D-xylulose 5-phosphate + ADP + H+
In E. coli, XK forms part of the xylose metabolism pathway by converting xylulose to X5P, which is then further metabolised in the pentose phosphate pathway.
E. coli already exhibit XK genes.
Ultimately, this part serves to consolidate the efficiency of conversion from xylulose to X5P in E. coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 719
Illegal AgeI site found at 1007 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
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