Composite
XK

Part:BBa_K4324002

Designed by: Chris Yoo   Group: iGEM22_TheKingsSchool_AU_HS   (2022-10-02)
Revision as of 13:31, 2 October 2022 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


lacPO-RBS-XK-T1

This part allows E. coli to express xylulose kinase (XK).

XK is an enzyme that allows the conversion of xylulose to xylulose-5-phosphate (X5P), and vice versa, according to the following chemical equation: D-xylulose + ATP ⇌ D-xylulose 5-phosphate + ADP + H+

In E. coli, XK forms part of the xylose metabolism pathway by converting xylulose to X5P, which is then further metabolised in the pentose phosphate pathway.

E. coli already exhibit XK genes.

Ultimately, this part serves to consolidate the efficiency of conversion from xylulose to X5P in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 719
    Illegal AgeI site found at 1007
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None