Regulatory

Part:BBa_K4129001

Designed by: Giovanni Carlo Gendarini   Group: iGEM22_DTU-Denmark   (2022-10-02)
Revision as of 13:05, 2 October 2022 by GCG (Talk | contribs)


Promoter of the Saccharomyces cerevisiae FDH2 gene

The part consists of the 1000 bp upstream of Formate Dehydrogenase 2 (FDH2) CDS from Saccharomyces cerevisiae. FDH2 has been shown to be upregulated in response to furfural by transcriptomics data. The part was amplified from gDNA through PCR and used to test if the promoter responded to furfural in Aspergillus niger as well. When used to drive expression of mCherry in A. niger through transient expression. It is not active in A. niger or does not promote expression at levels high enough to be detected during transient expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 275
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None