Part:BBa_K4180003

snf1Δ381-633aa C-truncated

SNF1Δ381-633aa, C-terminus truncated from amino acid381 to 633 deleting autoinhibitory domain and SIP-interacting domain (SIR) Sequence and Features

        BBa K4180003 snf1Δ381-633aa
        PCR product on lane 3 compared to
        BBa K4180002 snf1Δ2-306aa
         PCR product on lanes 1 and 2

After Bacteria transformation, bacteria colonies were picking up directly to do snf1Δ2-306aa - colonies had BBa_K4180002 snf1Δ2-306aa
         PCR product

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 528
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]