Coding

Part:BBa_K4180003

Designed by: HuiChing Kuo   Group: iGEM22_KCIS_Xiugang_Taipei   (2022-08-26)
Revision as of 08:50, 2 October 2022 by TedChueh (Talk | contribs)


snf1Δ381-633aa C-truncated

SNF1Δ381-633aa, C-terminus truncated from amino acid381 to 633 deleting autoinhibitory domain and SIP-interacting domain (SIR) Sequence and Features

        BBa K4180003 snf1Δ381-633aa
        PCR product on lane 3 compared to
        BBa K4180002 snf1Δ2-306aa
         PCR product on lanes 1 and 2

After Bacteria transformation, bacteria colonies were picking up directly to do snf1Δ2-306aa - colonies had BBa_K4180002 snf1Δ2-306aa
         PCR product



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 528
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
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