Coding

Part:BBa_K4180002

Designed by: HuiChing Kuo   Group: iGEM22_KCIS_Xiugang_Taipei   (2022-08-26)
Revision as of 08:30, 2 October 2022 by TedChueh (Talk | contribs)


snf1Δ2-306aa N-truncated

snf1Δ2-306aa, N-terminus truncated from amino acid 2 to 306 deleting kinase domain of SNF1, where the phosphorylation takes place on Thr210 to activate the catalytic activity of SNF1 Sequence and Features

     BBa K4180002
    snf1Δ2-306aa          PCR product

After Bacteria transformation, bacteria colonies were picking up directly to do SNF1 full length PCR - colonies #1, 3, 5, and 6 had BBa_K4180000(SNF1 full length)
         PCR product



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None