Regulatory

Part:BBa_K4129000:Design

Designed by: Giovanni Carlo Gendarini   Group: iGEM22_DTU-Denmark   (2022-10-01)
Revision as of 15:52, 1 October 2022 by Registry (Talk | contribs)

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Promoter of the Saccharomyces cerevisiae FDH1 gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 278
    Illegal XhoI site found at 519
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 193
    Illegal BsaI.rc site found at 345


Design Notes

Since we do not know what motifs are responsible for the promoter being upregulated by furfural in S. cerevisiae, we did not modify the sequence at all (even leaving BsaI sites)


Source

The part was obtained from gDNA

References