Composite
PcusC-GFP

Part:BBa_K4385011:Design

Designed by: Min An Zhao   Group: iGEM22_Jilin_China   (2022-10-01)
Revision as of 13:27, 1 October 2022 by Minan (Talk | contribs) (→‎Design Notes)


Lead sensor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 462
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 462
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 462
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 462
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 253
    Illegal BsaI.rc site found at 1394


Design Notes

This part is composed of CusSR two-component signal transport system, PcusC and OmpC-CBP. When the zinc ion concentration in the environment exceeds a certain concentration, the PcusC promotes the transcription of downstream OmpC-CBP, and realizes the adsorption of copper ions in the environment. The 6*His Tag was added to this sequence to purify the recombinant protein.

Source

It is amplified from E. coli genomic DNA.

References