Composite
Part:BBa_K4408007
Designed by: Leyu Xu Group: iGEM22_Worldshaper-NJBIOX (2022-09-29)
Lipase CALB connected with chitin binding domain
This part is the coding region of the fusion protein of Candida antarctica: lipase B (CALB) and chitin-binding domain (ChBD). It consists CALB (BBa_K2302006), Linker 2 (BBa_K4408004) and ChBD BBa_T2028) sequences. Candida antarctica lipase B (CALB) is a widely used lipase with high stereoselectivity and stability. ChBD is an affinity tag for chitin purification of proteins. ChBD-fusion CALB can facilitate the purification of CALB, thus enhancing the reaction rate of CALB catalyzed esterification.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 949
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 670
Illegal NgoMIV site found at 780
Illegal AgeI site found at 472
Illegal AgeI site found at 730 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1025
Illegal SapI site found at 964
Illegal SapI site found at 994
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Categories
Parameters
None |