Composite

Part:BBa_K4408007

Designed by: Leyu Xu   Group: iGEM22_Worldshaper-NJBIOX   (2022-09-29)
Revision as of 11:50, 1 October 2022 by Xuleyu (Talk | contribs)


Lipase CALB connected with chitin binding domain

This part is the coding region of the fusion protein of Candida antarctica: lipase B (CALB) and chitin-binding domain (ChBD). It consists CALB (BBa_K2302006), Linker 2 (BBa_K4408004) and ChBD BBa_T2028) sequences. Candida antarctica lipase B (CALB) is a widely used lipase with high stereoselectivity and stability. ChBD is an affinity tag for chitin purification of proteins. ChBD-fusion CALB can facilitate the purification of CALB, thus enhancing the reaction rate of CALB catalyzed esterification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 949
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 670
    Illegal NgoMIV site found at 780
    Illegal AgeI site found at 472
    Illegal AgeI site found at 730
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1025
    Illegal SapI site found at 964
    Illegal SapI site found at 994


[edit]
Categories
Parameters
None