Device

Part:BBa_K4408010:Design

Designed by: Leyu Xu   Group: iGEM22_Worldshaper-NJBIOX   (2022-09-29)
Revision as of 11:29, 1 October 2022 by Xuleyu (Talk | contribs) (→‎Design Notes)


Pthl-adhE2-Spythl-Spyhbd, Expression of adhE2, thl and hbd, modified by SpyCatcher/SpyTag oligopept


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4292
    Illegal SapI.rc site found at 4116


Design Notes

After producing the precursors from Clostridium tyrobutyricum, we produced CALB enzyme in E.coli to improve the rate of esterification. To further enhance the reaction rate, we introduced ChBD to purify the CALB enzyme.

Source

1

References