Device

Part:BBa_K4408009

Designed by: Leyu Xu   Group: iGEM22_Worldshaper-NJBIOX   (2022-09-29)
Revision as of 11:08, 1 October 2022 by Xuleyu (Talk | contribs)


Pthl-adhE2-thl-hbd, Expression of adhE2, thl and hbd with Pthl promoter

This part is responsible for expressing adhE2, thl and hbd proteins. The expression of these proteins are simultaneously controlled by a Pthl promoter with a ribosome binding site (RBS) and a Cpa fdx terminator. It consists of BBa_K3443002(Pthl), BBa_K103015(RBS1), BBa_K1462060(adhE2), BBa_K3443003 (thl), BBa_M1350 (hbd) and BBa_K3585002(Cpa fdx terminator).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3907
    Illegal SapI.rc site found at 3731


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Categories
Parameters
None