Composite
Part:BBa_K4385010
Designed by: Min An Zhao Group: iGEM22_Jilin_China (2022-10-01)
Heavy metal detoxification protein
The copper ion sensor part is composed of PcusC and CusSR two-component membrane associated sensor kinase system. CusSR is composed of membrane-associated histidine kinase CusS and transcription activator CusR, responsible for sensing the presence of external Cu2+. When external copper is sensed, the transmembrane structure of CusS protein undergoes a conformational change, and at the same time, autophosphorylation occurs to transmit the signal into the cell. The phosphoryl group is then transferred to the CusR protein, triggering a conformational change in CusR, and the phosphoryl group binds to a sequence in front of the PcusC promoter to turn on downstream gene expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 142
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |