Part:BBa_K200005

OtsA: Part 1 of 2 for trehalose producing enzymes.

Sequence codes for trehalose-6-phosphate synthase enzyme. This enzyme is the first of two required in the conversion of glucose to trehalose.
This enzyme catalyses the following reaction:
UDP-glucose + D-glucose 6-phosphate -> UDP + alpha,alpha-trehalose 6-phosphate

Trehalose is a disaccharide formed from two glucose molecules. Throughout nature, trehalose is associated with resistance to dessication and cold shock otsa1, and is naturally produced in Escherichia Coli. We hope that by upregulating the trehalose production pathways in E.coli we can increase trehalose concentrations within our cell, thereby conferring some resistance to protein degredation in our system. This would allow easy transport and storage of the final product.

Usage and Biology

Figure 1: Trehalose (source: [http://en.wikipedia.org/wiki/Trehalose wikipedia])

Trehalose (α,α-trehalose) is contains two D-glucose units in an α,α-1,1 linkage. [http://www.cababstractsplus.org/abstracts/Abstract.aspx?AcNo=20023107854 [1]] It is a non-reducing sugar.

Properties of trehalose:

[http://www.iupac.org/publications/pac/2002/pdf/7407x1263.pdf [2]]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 383
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

<biblio>#otsa1 pmid=12105274 </biblio>