Part:BBa_K200012

lambda promoter (cIts responsive)

This is different from the common lambda promoter in that it is able to be repressed by the temperature sensitive cI protein (BBa_K200011).

This lambda promoter contains 3 operator sites, as opposed to only 2 operator sites on the registry lambda promoter (BBa_R0051)

It has been shown through lysis experiments pL1 that this lambda promoter can be stringently repressed by the cI protein at temperatures up to 30°C. At temperatures higher than 30°C, the cI repressor is progressively thermally denatured and no longer able to bind to the promoter operator regions. Therefore, promoter activity is induced.

The system of the lambda promoter- temperature sensitive cI protein provides an effective way to regulate gene expression using temperature.

Requirements/Limitations

• Possible crosstalk with other lambda repressors

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

<biblio> pL1 pmid=10227165 </biblio>