Part:BBa_I750016:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

User Reviews

21 May 2009

Phillip, Randy,

I'm writing with a question about BBa_I750016, Gas Vesicle polycistonic gene, as entered in the Registry of Standard Biological Parts:

https://parts.igem.org/Part:BBa_I750016

The long description states that the parts is ~5.7 kb long.

The sequence stored in the Registry is 6064 bp long

From examination, their appear to be 11 perfect direct repeats starting with "tctgcaaatta" in the DNA sequence stored in the Registry.

I couldn't find any annotated feature associated with such direct repeats, and thus wonder whether there is a mistake in the DNA sequence that the Registry is providing.

I have added this email to the parts experience page on the Registry.

Thank you, Drew

____

Drew Endy Stanford Bioengineering The BioBricks Foundation

2 September 2009

Drew,

we are currently working with BBa_I750016, Gas Vesicle polycistonic gene, as entered in the Registry of Standard Biological Parts:

https://parts.igem.org/Part:BBa_I750016

The plan is to remove the 10x 40bp (400bp total) repeat in gene gvpL, and thereby reduce the size of the biobrick to its original size of ~5.7 kb. In doing so, we also hope to remove the unwanted mutation next to the repeat section, and thus return the biobrick to its original form as in pNL29 without PstI and EcoRI sites.

As far as we could tell, there were no additional changes in the biobrick.

Besides the sequence, we are also trying to test the buoyancy of the brick, and caracterize it in this way.

From iGEM Groningen,

Michael Verhoeven

UNIQ4cc0645a55c60566-partinfo-00000000-QINU UNIQ4cc0645a55c60566-partinfo-00000001-QINU