![](https://parts.igem.org/images/partbypart/icon_plasmid.png)
Plasmid
Part:BBa_K4231019
Designed by: Shi Han Group: iGEM22_USTC (2022-09-30)
pUC19 carrying with ERG12, ERG20, BbTps3 and LEU tag from Y.lipolytica
The plasmid backbone pUC19 combined with pTef1-BbTps3, pGAP-ERG20, pTef1-ERG12, Leu tag. It can be transformed to Y.lipolytica so as to establish MVA pathway.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6096
Illegal EcoRI site found at 6146
Illegal EcoRI site found at 7108
Illegal XbaI site found at 5583
Illegal SpeI site found at 1468
Illegal PstI site found at 1285
Illegal PstI site found at 2071
Illegal PstI site found at 2826
Illegal PstI site found at 3406
Illegal PstI site found at 3796
Illegal PstI site found at 5209 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6096
Illegal EcoRI site found at 6146
Illegal EcoRI site found at 7108
Illegal SpeI site found at 1468
Illegal PstI site found at 1285
Illegal PstI site found at 2071
Illegal PstI site found at 2826
Illegal PstI site found at 3406
Illegal PstI site found at 3796
Illegal PstI site found at 5209 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6096
Illegal EcoRI site found at 6146
Illegal EcoRI site found at 7108
Illegal BglII site found at 812
Illegal BglII site found at 944
Illegal BglII site found at 8377
Illegal BamHI site found at 2447
Illegal BamHI site found at 5528
Illegal XhoI site found at 1073
Illegal XhoI site found at 1229
Illegal XhoI site found at 3979
Illegal XhoI site found at 7091
Illegal XhoI site found at 7120 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6096
Illegal EcoRI site found at 6146
Illegal EcoRI site found at 7108
Illegal XbaI site found at 5583
Illegal SpeI site found at 1468
Illegal PstI site found at 1285
Illegal PstI site found at 2071
Illegal PstI site found at 2826
Illegal PstI site found at 3406
Illegal PstI site found at 3796
Illegal PstI site found at 5209 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6096
Illegal EcoRI site found at 6146
Illegal EcoRI site found at 7108
Illegal XbaI site found at 5583
Illegal SpeI site found at 1468
Illegal PstI site found at 1285
Illegal PstI site found at 2071
Illegal PstI site found at 2826
Illegal PstI site found at 3406
Illegal PstI site found at 3796
Illegal PstI site found at 5209
Illegal NgoMIV site found at 2200
Illegal NgoMIV site found at 3081
Illegal NgoMIV site found at 3772
Illegal NgoMIV site found at 6234
Illegal AgeI site found at 3680
Illegal AgeI site found at 7705 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1966
Illegal BsaI.rc site found at 3872
Illegal BsaI.rc site found at 5826
Illegal SapI site found at 5179
[edit]
Categories
Parameters
None |