![](https://parts.igem.org/images/partbypart/icon_plasmid.png)
Plasmid
Part:BBa_K4231018:Design
Designed by: Shi Han Group: iGEM22_USTC (2022-09-30)
pUC19 carrying with IDI, NADH-HMG, ERG8, ERG19 and URA3 tag from Y.lipolytica
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2325
Illegal EcoRI site found at 8712
Illegal PstI site found at 862
Illegal PstI site found at 1992
Illegal PstI site found at 7315 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2325
Illegal EcoRI site found at 8712
Illegal NheI site found at 634
Illegal PstI site found at 862
Illegal PstI site found at 1992
Illegal PstI site found at 7315 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2325
Illegal EcoRI site found at 8712
Illegal BglII site found at 719
Illegal BglII site found at 6238
Illegal BamHI site found at 974
Illegal BamHI site found at 1073
Illegal BamHI site found at 1613
Illegal BamHI site found at 6936
Illegal BamHI site found at 8102
Illegal XhoI site found at 3028
Illegal XhoI site found at 3862
Illegal XhoI site found at 3895
Illegal XhoI site found at 6653 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2325
Illegal EcoRI site found at 8712
Illegal PstI site found at 862
Illegal PstI site found at 1992
Illegal PstI site found at 7315 - 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 7991
Illegal BsaI.rc site found at 2386
Illegal BsaI.rc site found at 3048
Illegal BsaI.rc site found at 3396
Illegal BsaI.rc site found at 7710
Illegal BsaI.rc site found at 8060
Design Notes
All parts were combined by restriction enzyme, T4 DNA ligase and infusion cloning.Considering homologous recombination in Y.lipolytica, we made homologous sequences pTef1 and pGAP as far apart as possible.
Source
The coding sequences of IDI, ERG8, ERG19, URA3 were amplified from Y.lipolytica.The ORF of NADH-HMG was found in NCBI and was synthesized according to the sequence.