Composite
Part:BBa_K4165040
Designed by: Salma Sobhy Sadek Group: iGEM22_CU_Egypt (2022-09-30)
HtrA1 Switch number 20
This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), pGS-21a RBS (BBa_K4165016), 6x His-tag (BBa_K4165020), WAP inhibitor (BBa_K4165008), GGGG linker (BBa_K4165067), TD28rev (BBa_K4165006), GGSGGGG linker (BBa_K4165018), WWW (BBa_K4165007), GGGG linker (BBa_K4165067), H1A (BBa_K4165000) and T7 terminator (BBa_K731721).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 379
Illegal AgeI site found at 115 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |