Part:BBa_K4147003
PcOSM Construct with LacI regulated promoter
This part contains the linear construct for pcOSM (plant osmotin from Piper colubrinum) with an included LacI promoter (1118 pb in total). Additionally, it contains an RBS under the part name BBa_B0032, as well as a 6X-His tag at the end of the osmotin CDS for later protein purification. Finally, it has a double terminator (BBa_B0015) to ensure correct termination. As a linear construct, it contains the RFC10 prefix and suffix, making the part compatible with other iGEM parts.
This osmotin is a plant defense protein with a molecular weight of ~24 kDa. PcOSM belongs to pathogenesis related-5 (PR-5) family, which is accumulated in response to both biotic and abiotic stresses [1]. This osmotin has exhibited significant inhibitory activity against the oomycete pathogen Phytophthora capsici. This protein is able to inhibit fungal growth through inhibition of hyphal growth, spore germination, spore lysis, and reduction in viability of spore [2].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 806
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 498
- 1000COMPATIBLE WITH RFC[1000]
| None |