Composite
Part:BBa_K4115043:Design
Designed by: Kaijun Wang Group: iGEM22_ShanghaiTech_China (2022-09-30)
lac UV5 promoter-nifA-PnifH-sfGFP-B0015
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 82
Illegal PstI site found at 223
Illegal PstI site found at 1087 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 223
Illegal PstI site found at 1087 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 724
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 82
Illegal PstI site found at 223
Illegal PstI site found at 1087 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 82
Illegal PstI site found at 223
Illegal PstI site found at 1087
Illegal NgoMIV site found at 1344
Illegal NgoMIV site found at 1539
Illegal NgoMIV site found at 1614
Illegal AgeI site found at 973 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1182
Illegal BsaI.rc site found at 2101
Illegal SapI site found at 405
Illegal SapI site found at 1026
Design Notes
none
Source
none