Composite
Part:BBa_K4268019
Designed by: J. Aubrey, J. Alvarenga, L. Buchanan, J. Reyes, D. Yashinski Group: iGEM22_SUNY_Oneonta (2022-09-30)
S-TIP 37 Ghost Phagemid
This is the complete cyanophage phagemid with multi-transcription units of Coding Sequence Expression Cassette 1 and Coding Sequence Expression Cassette 3.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 3810
Illegal PstI site found at 7463 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 6101
Illegal NheI site found at 10637
Illegal PstI site found at 3810
Illegal PstI site found at 7463 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 5114
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 3810
Illegal PstI site found at 7463 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 3810
Illegal PstI site found at 7463
Illegal NgoMIV site found at 3450
Illegal NgoMIV site found at 3589
Illegal NgoMIV site found at 4545
Illegal NgoMIV site found at 12134
Illegal AgeI site found at 14165 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |