Composite

Part:BBa_K4268018:Design

Designed by: J. Aubrey, J. Alvarenga, L. Buchanan, J. Reyes, D. Yashinski   Group: iGEM22_SUNY_Oneonta   (2022-09-30)
Revision as of 00:24, 1 October 2022 by Registry (Talk | contribs)

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Biotin Tagged Capsid and Core Multi-Transcriptional Unit


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3806
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 3806
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5170
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3806
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3806
    Illegal NgoMIV site found at 3446
    Illegal NgoMIV site found at 3585
    Illegal NgoMIV site found at 4541
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Golden Gate Assembly Level 2



Source

The coding sequences were derived from the S-TIP37 genome.


References