Coding
Part:BBa_K4323002:Design
Designed by: Jessica Marinelli, Kevin Lim, Chun Hin Wong Group: iGEM22_UManitoba (2022-09-30)
Truncated FitD
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 909
Illegal BamHI site found at 1019
Illegal BamHI site found at 2568 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2191
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We use bioinformatics tools and literature to determine possible regions to truncate and to keep. Since most transmembrane domains are located within the core 1300-2200 bp, we decide to use this as our design.
Source
Pseudomonas protegens