Device

Part:BBa_K4364000:Design

Designed by: Slavil Peykov   Group: iGEM22_Bulgaria   (2022-09-30)
Revision as of 22:54, 30 September 2022 by Registry (Talk | contribs)

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mRFP1-based cassette for TA cloning with dual T7 promotors


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 199
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 207
    Illegal BamHI site found at 316
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 906
    Illegal AgeI site found at 1018
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Be careful that other parts of your construct and/or the vector backbone do not contain AhdI recognition sites.


Source

IDT synthesis - gBolck

References