Composite

Part:BBa_K4387003

Designed by: Jana Mehdy, Lea Bruellmann, Marine Mausy   Group: iGEM22_UZurich   (2022-09-30)
Revision as of 21:20, 30 September 2022 by Registry (Talk | contribs)

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Negative Control of Nitric Oxide Sensing Genetic Circuit

In order to test the exact effect of our construct, we removed the PnorV_beta promoter to create a negative control. With the removal of the promoter, no NorR should be produced; thus, the promoter will not be activated, and no GFP will be produced.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 439
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None