Coding

Part:BBa_K200005

Designed by: Royah Vaezi, David Roche   Group: iGEM09_Imperial College London   (2009-08-12)
Revision as of 14:02, 1 September 2009 by Droche (Talk | contribs)

OtsA: Part 1 of 2 for trehalose producing enzymes.

Sequence codes for trehalose-6-phosphate synthase enzyme. This catalyses the following reaction:
UDP-glucose + D-glucose 6-phosphate -> UDP + alpha,alpha-trehalose 6-phosphate
This enzyme is the first of two required in the conversion of glucose to trehalose.

Trehalose is a disaccharide formed from two glucose molecules. Throughout nature, trehalose is associated with resistance to dessication and cold shock [http://www.pnas.org/content/99/15/9727.full.pdf+html 1], and is naturally produced in Escherichia Coli. We hope that by upregulating the trehalose production pathways in E.coli we can increase trehalose concentrations within our cell, thereby conferring some resistance to protein degredation in our system. This would allow easy transport and storage of the final product.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 383
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None