Coding

Part:BBa_K200006:Design

Designed by: Royah Vaezi, David Roche   Group: iGEM09_Imperial College London   (2009-08-12)
Revision as of 13:13, 1 September 2009 by Droche (Talk | contribs) (→‎Design Notes)

OtsB: Part 2 of 2 coding for trehalose producing enzymes


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 455
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR from Escherichia Coli BL21(DE3)

Primers Used:
Forward:
GCTCTAGATGACAGAACCGTTAACCG
Recomended Temperatures for PCR : 54.5C(without overhang) and 64.8C(with overhang)

Reverse:
GGACTAGTATTAGATACTACGACTAAACGAC
Recomended Temperatures for PCR : 54.7C(without overhang) and 64.2C(with overhang)

Biobrick Overhang shown in Bold

Source

Coding sequence isolated from e-coli

References