Composite

Part:BBa_K200009

Designed by: David Roche, Royah Vaezi   Group: iGEM09_Imperial College London   (2009-08-12)
Revision as of 12:35, 1 September 2009 by Dineka (Talk | contribs) (→‎References)

DpnII Composite

DpnII is a dam sensitive restriction enzyme.
The recognition sequence of this restriction enzyme is GATC and is a 4 base cutter, leaving a 4 base overhang.


The cutting site is as shown:
NGATCN -> N / GATCN
NCTAGN -> NCTAG / N


As part of the Imperial 2009 iGEM E.ncapsulator project, DpnII was used to cut up the genetic material within the cell, completing the cell death module.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[http://www.neb.com/nebecomm/products/productR0543.asp Information from NEB]



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