Coding

Part:BBa_K4229021

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-09-28)
Revision as of 19:00, 30 September 2022 by NikitaEdel (Talk | contribs)


sfGFP AMBER stop codon at the position of the 4th amino acid 


Superfolder GFP (sfGFP) with an amber stop codon mutation at position 8, replacing phenylalanine

Short Description:

The F8 variant of sfGFP was created using site-directed mutagenesis, aiming to insert the amber stop codon at position 8. The sfGFP can only be completely synthesized upon successful amber stop codon suppression. Hence, sfGFP F8 can be used as a model to test the efficiency of incorporation of non-canonical amino acids via amber stop codon suppression technology.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 421
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None