Regulatory

Part:BBa_K4207016:Design

Designed by: Jesper Mickos   Group: iGEM22_Aboa   (2022-09-30)
Revision as of 18:08, 30 September 2022 by Registry (Talk | contribs)

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BYDV toehold switch B1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This toehold switch was designed according to the B-series toehold structure (Pardeee et. al., 2016). The A-series structure was designed to reduce translational leakage compared to older toehold switches (Green et. al., 2014). The binding site of the toehold switch was designed to bind to a conserved sequence in the BYDV genome, allowing it to sense the presence of this trigger sequence. This toehold has a downstream 21-nt linker sequence, which ends in the codon AAG. To prevent frame-shifting, the last G is omitted from the documented sequence and the junction site should have the sequence GATG instead of the standard AATG junction site.


Source

This part was created by us, using 10 different barley yellow dwarf virus (BYDV) whole genomes to screen for conserved sequences to be detected by the toehold switches.

References