Regulatory

Part:BBa_K4159002:Design

Designed by: Melissa Hendrén   Group: iGEM22_Aalto-Helsinki   (2022-07-14)
Revision as of 17:24, 30 September 2022 by VeeraKurki (Talk | contribs) (→‎Design Notes)


T7 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 21
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 21
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 21
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The T7 promoter with the added stem-loop had been used in the plasmid (pBx1-VHH_template3-3XMyc-Spacer) [1], no additional changes were needed the reference sequence. The XbaI cutting site is in the stem-loop, and as the part was used in the DARPin construct fragment this was not an issue. test.

Source

[1] Chen X, Gentili M, Hacohen N, Regev A. A cell-free nanobody engineering platform rapidly generates SARS-CoV-2 neutralizing nanobodies. Nat Commun. 2021 Sep 17;12(1):5506. doi: 10.1038/s41467-021-25777-z. PMID: 34535642; PMCID: PMC8448731.

References