Composite

Part:BBa_K200009

Designed by: David Roche, Royah Vaezi   Group: iGEM09_Imperial College London   (2009-08-12)
Revision as of 14:19, 28 August 2009 by Dineka (Talk | contribs)

DpnII Composite

Dam Sensitive Restriction Enzyme. The recognition sequence of this restriction enzyme is GATC, and this enzyme is a 4 base cutting enzyme leaving a 4 base overhang.



The Cutting Site is as shown:
NGATCN -> N / GATCN
NCTAGN -> NCTAG / N


As part of the Imperial 2009 iGEM E.ncapsulator project, DpnII was used to cut up the genetic material within the cell, completing the cell death module.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None