Part:BBa_K4140004

P2A linker

Part Description

In order to mediate the "cleavage" of two proteins, 2A peptide sequences were discovered in Picornaviruses. Effective multiple protein expression from a single open reading frame (ORF) is achieved using 2A peptide-linked multicistronic vectors. Use alternative 2A peptide sequences when linking more than two genes to reduce the likelihood of homologous recombination. All eukaryotic systems studied to date, including mammalian cells, yeast, and plants, have effectively utilised the 2A peptide system.

Usage

Before implementing our therapeutic circuit product into a human body, we employed this 2A linker to attach a reporter gene to it so that we could test its functionality and ability to express PAH by utilizing spectrophotometry to monitor the degree of PAH expression.

Literature Characterization

Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs.

References

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]