Composite

Part:BBa_K4183004

Designed by: Junjie Zhu   Group: iGEM22_HS_China   (2022-09-30)
Revision as of 16:12, 30 September 2022 by Registry (Talk | contribs)

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Testing the synthesis efficiency of bacterial surface capsular polysaccharide (CAP).

CAP is a non-toxic podoconjugate polysaccharide derived from E. coli Nissle 1917 (EcN) for use in tumor therapy in vivo. This component was used to test the efficiency of CAP synthesis in EcN.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 433
    Illegal PstI site found at 1565
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 433
    Illegal PstI site found at 1565
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 433
    Illegal BglII site found at 1015
    Illegal BamHI site found at 1368
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 433
    Illegal PstI site found at 1565
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 433
    Illegal PstI site found at 1565
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None