Coding

Part:BBa_K200008

Designed by: Charles Fracchia, Royah Vaezi, Tianyi Wang, Nuri Purswani   Group: iGEM09_Imperial College London   (2009-08-12)
Revision as of 10:38, 28 August 2009 by Nuri (Talk | contribs)

Phenylalanine hydroxylase


[http://en.wikipedia.org/wiki/Phenylalanine_hydroxylase Phenylalanine hydroxylase (PAH)] is the enzyme that determines the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.#PAH1

As part of the Imperial 2009 iGEM E.ncapsulator project, E.coli was engineered to synthesise PAH to a tunable threshold, and successfully coat and protect it through the stomach, until release in the small intestine.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 287
    Illegal BamHI site found at 814
    Illegal XhoI site found at 524
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



References

[http://www.ncbi.nlm.nih.gov/nuccore/NM_000277.1?report=fasta Sequence from NCBI] <biblio>#PAH1 pmid=2014036 </biblio>

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Categories
Parameters
None