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Coding
Part:BBa_K4212010
Designed by: Youzi Bi Group: iGEM22_Imperial_College_London (2022-09-30)
GerAK_modified_chimera
Synthetic operon designed with GerAA, GerKB and GerAC coding sequences from the B. subtilis genome. Designed to be transformed in a B. subtilis knockout strain so as to produce a germinant receptor that contains a binding site for glucose, and when activated triggers an orthogonal signalling cascade resulting in germination. It is an improved version of the GerAK chimera.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 992
Illegal EcoRI site found at 1308
Illegal PstI site found at 943
Illegal PstI site found at 2762 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 992
Illegal EcoRI site found at 1308
Illegal PstI site found at 943
Illegal PstI site found at 2762 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 992
Illegal EcoRI site found at 1308
Illegal BglII site found at 524
Illegal XhoI site found at 2869 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 992
Illegal EcoRI site found at 1308
Illegal PstI site found at 943
Illegal PstI site found at 2762 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 992
Illegal EcoRI site found at 1308
Illegal PstI site found at 943
Illegal PstI site found at 2762 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 179
[edit]
Categories
Parameters
None |