Regulatory

Part:BBa_K4263001

Designed by: Hanlin Li   Group: iGEM22_SCUT-China   (2022-09-24)
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K4263001

PACC1

Introduction

ACC1 is the major member of ACCs in mammalian[1].

Characterization

In order to test the function of PACC1, we construct "PACC1-EGFP-terminator" (Figure 1). If PACC1 is functional, we can test the fluorescence intensity of EGFP in supernatant samples obtained from the culture of recombinant P.pastoris GS115 strain.

Figure 1 Gene circuit of PACC1-EGFP-terminator

Our results matched the general expected trend (Figure 2). After fermentation experiment in BMMY medium containing 1% methanol. The fluorescence intensity of the samples of recombinant P.pastoris GS115 containing the EGFP gene gradually increased over time . At the same time, we measured the growth curve of the strains.

Figure 2 Fluorescence intensity and OD600 absorbance of samples obtained at different time points from the culture of corresponding recombinant P.pastoris GS115 containing EGFP gene.

Reference

[1] Wang Y, Yu W, Li S, et al. Acetyl-CoA Carboxylases and Diseases[J]. Front Oncol, 2022,12:836058.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 365



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