Coding

Part:BBa_K4241018

Designed by: Ng Tsz Chun   Group: iGEM22_HKU_HongKong   (2022-09-30)
Revision as of 15:17, 30 September 2022 by Registry (Talk | contribs)

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Mxe_GyrA_Intein with PT linker and ELK16

This can be fused to a target protein for inclusion expression, later on could have DTT mediated cleavage to purify the protein. This is a mutant version.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6173929/


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 676
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 499
    Illegal NgoMIV site found at 514
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None