Composite

Part:BBa_K4307006

Designed by: Chi Zhang   Group: iGEM22_Tsinghua   (2022-09-30)
Revision as of 15:07, 30 September 2022 by Registry (Talk | contribs)

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attB-promoter-attP & Ser integrase

Serine integrase, adapted from bacteriophage, is capable of catalyzing site-specific recombination between two attachment sites (attP and attB) and inverting the DNA sequence flanked by these two opposing sites. We use Bxb1 integrase, an integrase found in Mycobacteriophage Bxb1 in our project.

This time, we combined attP and attB, the attachment sites of serine integrase with OR1-OR2, the part that has promoter function in the right operator (OR) of phage genome.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 361
    Illegal PstI site found at 667
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 361
    Illegal NheI site found at 350
    Illegal PstI site found at 667
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 361
    Illegal BglII site found at 525
    Illegal BamHI site found at 289
    Illegal BamHI site found at 549
    Illegal BamHI site found at 927
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 361
    Illegal PstI site found at 667
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 361
    Illegal PstI site found at 667
    Illegal NgoMIV site found at 4
    Illegal NgoMIV site found at 1266
    Illegal AgeI site found at 124
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 24
    Illegal BsaI.rc site found at 1933
    Illegal SapI site found at 106


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