![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4307006
Designed by: Chi Zhang Group: iGEM22_Tsinghua (2022-09-30)
attB-promoter-attP & Ser integrase
Serine integrase, adapted from bacteriophage, is capable of catalyzing site-specific recombination between two attachment sites (attP and attB) and inverting the DNA sequence flanked by these two opposing sites. We use Bxb1 integrase, an integrase found in Mycobacteriophage Bxb1 in our project.
This time, we combined attP and attB, the attachment sites of serine integrase with OR1-OR2, the part that has promoter function in the right operator (OR) of phage genome.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 361
Illegal PstI site found at 667 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 361
Illegal NheI site found at 350
Illegal PstI site found at 667 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 361
Illegal BglII site found at 525
Illegal BamHI site found at 289
Illegal BamHI site found at 549
Illegal BamHI site found at 927 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 361
Illegal PstI site found at 667 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 361
Illegal PstI site found at 667
Illegal NgoMIV site found at 4
Illegal NgoMIV site found at 1266
Illegal AgeI site found at 124 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 24
Illegal BsaI.rc site found at 1933
Illegal SapI site found at 106
[edit]
Categories
Parameters
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