Composite

Part:BBa_K4220100

Designed by: Xiaoya Liu   Group: iGEM22_NEU_CHINA   (2022-09-30)
Revision as of 14:04, 30 September 2022 by Registry (Talk | contribs)

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HSF1up-HIS3+NatR+ADH1+CUP1-HSF ORF

This is a kill switch in yeast which can let the yeast survive in the presence of copper ions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 247
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 367
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 512


[edit]
Categories
Parameters
None