Composite

Part:BBa_K4229066:Design

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-09-30)
Revision as of 12:11, 30 September 2022 by Registry (Talk | contribs)

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mVenus with N-terminal spyCatcher regulated by tetA/B promotor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 59
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 59
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 59
    Illegal BglII site found at 68
    Illegal XhoI site found at 1075
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 59
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 59
    Illegal AgeI site found at 508
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

Was kindly send to us by the Kerfeld group. From this paper https://doi.org/10.1073/pnas.2116871119


References