Composite

Part:BBa_K4273019

Designed by: Su Junzhe   Group: iGEM22_LINKS_China   (2022-09-30)
Revision as of 11:57, 30 September 2022 by Registry (Talk | contribs)

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pTDH3-Np5598-tTDH1-pPGK1-Np5597-tPGK1

Gene from cyanobacteria N. punctiforme. involved in biosynthesis of shinorine.

encoding ATP-grasp ligase(AG-L) that converts 4-deoxygadusol (4-DG) to mycosporine-glycine(MG) and D-Ala-D-Ala ligase(ALA-L) that converts mycosporine-glycine(MG) to Shinorine.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3423
    Illegal XhoI site found at 2075
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2321
    Illegal BsaI.rc site found at 3433


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Parameters
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