Composite

Part:BBa_K4273018

Designed by: Su Junzhe   Group: iGEM22_LINKS_China   (2022-09-30)
Revision as of 11:49, 30 September 2022 by Registry (Talk | contribs)

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pTDH3-EEVS-tTDH1-pPGK1-MTOx-tPGK1

Gene from zebrafish（Danio rerio）involved in biosynthesis of gadusol and gadusolate .Encoding 2-epi-5-epi-valiolone synthase (EEVS) That converts sedoheptulose 7-phosphate (S7P)to 2-epi-5-epi-valiolnoe (EEV) and MT-Ox that converts 2-epi-5-epi-valiolnoe (EEV) to gadusol and gadusolate

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1202
    Illegal BglII site found at 1456
    Illegal BglII site found at 3450
    Illegal XhoI site found at 2102
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1384
    Illegal AgeI site found at 1990
    Illegal AgeI site found at 3750
    Illegal AgeI site found at 3972
    Illegal AgeI site found at 4401
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2348
    Illegal BsaI.rc site found at 3460


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Categories
Parameters
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